At the moment, the early symptoms had been dismissed as debris, though the retrospective evaluation shown shared features Along with the problematic cells in culture. Tracing the infection helped the lab establish added laboratory strategies for screening and safety of assay-vital mobile lines.
Regardless of whether you’re a novice or an authority, check out our resources to advance your exploration and expertise in flow cytometry.
Mix acoustic focusing technologies with higher-parameter Assessment for adaptable spectral unmixing and conventional workflows.
The award-profitable Invitrogen Attune CytPix Flow Cytometer combines acoustic focusing with a higher-velocity brightfield camera for simultaneous high throughput flow cytometry and large resolution brightfield imaging.
"A picture isn't very worthy of one thousand dots, nonetheless it truly helps to see images. It is great to determine photographs that correlate with staining. One example is, AnnexinV-constructive cells that are morphologically various than nutritious cells, or CD14+ monocytes which are much larger and more textured than CD3+/CD4+/CD14- lymphocytes.
Listed here, a highly trained user has gated singlets confidently. Soon after analyzing the manual singlet gate, the CytPix image-derived parameter ParticleCount reveals this gate incorporates in excess of 4% aggregates.
Pair your Attune flow cytometer with an autosampler for improved workflow efficiency, and insert robotic integration for maximum throughput.
Our researchers will style panels to suit your needs as again and again you would like—cost-free, no product or service purchase needed.
Early signs of this contamination had at first been dismissed as debris. Backgating to discover new populations. Backgating imaged cells over the Attune CytPix Flow Cytometer also means that you can use morphological characteristics to find out website fascinating subpopulations that might not be clear from flow cytometry knowledge by yourself.
People can check out function illustrations or photos in serious-time to confirm sample excellent, enhance protocols, and record the morphology of every mobile inhabitants determined for Examination. Imaging seize also Added benefits from acoustic concentrating, which really helps to placement the cells in order that a pointy, centered image is obtained.
AI-pushed automated impression Evaluation While using the Attune Cytometric Application translates function features into distinctive morphology parameters which can be coupled with standard fluorescence and scatter parameters to expose highly reproducible inhabitants adjustments and exceptional subsets.
We Formerly demonstrated the power of imaging CAR-T/Ramos mobile interactions. Allow’s evaluate just the population of best desire, the double positive events, To find out more. We can easily now use prolonged morphological parameters (right here, circularity and skewness of depth) to further more analyze the functions of these populations and refine gating on these events, growing facts robustness.
Leverage the power of Innovative know-how and innovation to speed up your discoveries. Have confidence in inside the Attune Flow Cytometer loved ones to deliver the dependability, velocity, and precision that established your perform apart.
No wash/no lyse leukocyte Evaluation with imaging. Cells were acquired from 24-hour-aged blood by dilution in 1 mM EDTA ( Specialist UX/UI enables end users to practice AI versions for expanded applications. (A) The “Educate Product” function presents annotation applications and serious-time model schooling accuracy.
At the center of each and every Attune Flow Cytometer lies our revolutionary acoustic focusing technology, which makes use of seem waves to meticulously align cells within the flow cell. This progressive approach surpasses standard hydrodynamic strategies, leading to speedier processing speeds, exceptional details quality, and greater sample throughput.
Since the cell line had Earlier been analyzed about the Attune CytPix Flow Cytometer, the researchers went back to the photographs and ended up in a position to document the microbial an infection at the very least five days earlier.
Agilent Price Assure ensures you no less than 10 years of instrument use within the date you buy. Otherwise, we will credit you Together with the residual value of the program toward an upgraded model.
What seemed to be a homogeneous population is in fact additional numerous—an insight that needs to be deemed when interpreting benefits.